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Presented at the Pacific Coast Reproductive Society Meeting.
April, 1995. Rancho Mirage, CA
A Novel One Step Sperm Wash Product
JE Ellington, RW Wright, S Broder 1, AJ Benson, & SA Oliver
INGfertility, Spokane, WA & 1California Cryobank, Los Angeles, CA
A continuous gradient sperm wash product containing arabinogalactan, Sperm Concept (now sold as IsoCare One Step) was compared against a Percoll wash in 3 experiments.
Experiment 1: In Expt. 1, fresh semen from 4 men was divided and washed either in SC or 80% Percoll (P) for 30” at 300 x g. Pellets were then washed again in media alone and sperm evaluated. All data are expressed below as mean SEM for SC versus P. There was no difference in the % motility for recovered sperm (83 4% vs 83 3%), the motility of sperm cultured for 24 h (64 6% vs 75 6%) or in the numbers of sperm recovered (p=0.6). Based on animal model data showing a lack of toxicity of SC to sperm throughout the IVF process, the next 2 experiments were done on sperm removed directly from the SC pellet, without a second media wash step.
Experiment 2: In Expt. 2, SC was compared to bilayer P (90%:45%), to wash fresh semen from 8 men. The % normal morphology (83% 3% vs 81 1%) and motile sperm (74 6% vs 76 3%) did not differ between treatments. Both treatments improved (p<0.09) these parameters over that found for prewashed sperm morphology (72 2%) and motility (62 5%). The % of motile sperm recovered tended to be higher (p=0.1) after SC wash (93 5% vs 80 5%).
Experiment 3: In Expt. 3, semen from 12 men with fertility disorders was evaluated after washing in SC or P. There was no difference in motility of recovered sperm (59 12% vs 60 16%); however, normal membrane function (hypo-osmotic swell test) was better (p=0.045) in SC (70 13% vs 46 10%). Hamster egg SPA was also done on sperm washed from 4 donors in both SC and P. There was no difference in % eggs penetrated (41 2% vs 40 2%) or in the penetration factor (0.48 0.02 vs 0.41 0.03). One man did show a 30% improvement using SC versus P. The SC product is made from a polysaccharide approved for human consumption. It offers a non-toxic one step wash alternative to Percoll.
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